European eel Anguilla anguilla individuals from high (Tiber river, Italy) and low pollution (Bolsena lake, Italy) environments

An European eel-specific microarray platform was developed to identify genes involved in response to pollutants A comparative analysis of gene expression was conducted between European eel Anguilla anguilla individuals from high (Tiber river, Italy) and low pollution (Bolsena lake, Italy) environments. Gene expression profiling was performed using an European eel-specific oligo-DNA microarray of 14,913 probes based on single-colour detection (Cyanine-3 only). Microarrays were scanned with Agilent scanner G2565BA (barcode on the left, DNA on the back surface, scanned through the glass) at a resolution of 5 microns; all slides were scanned twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%); the scanner software created a unique ID for each pair of XDR scans and saved it to both scan image files. Feature Extraction (FE) 9.5 used XDR ID to link the pairs of scans together automatically when extracting data. The signal left after all the FE processing steps have been completed is ProcessedSignal that contains the Multiplicatively Detrended, Background-Subtracted Signal.

为鉴定参与污染物应答过程的基因，研究人员开发了一款欧洲鳗鲡特异性微阵列（microarray）平台。本研究针对采自高污染环境（意大利台伯河）与低污染环境（意大利博尔塞纳湖）的欧洲鳗鲡（Anguilla anguilla）个体，开展了基因表达对比分析。基因表达谱分析采用一款包含14913个探针、基于单色彩检测（仅使用花青苷-3（Cyanine-3））的欧洲鳗鲡特异性寡核苷酸DNA微阵列（oligo-DNA microarray）完成。微阵列扫描使用安捷伦（Agilent）扫描仪G2565BA完成，扫描参数为：分辨率5微米，玻片左侧带有条形码，DNA附着于玻片背面，扫描需透过玻片完成；所有芯片玻片均以两种不同灵敏度设置（XDRHi 100%与XDRLo 10%）分别扫描两次；扫描仪软件会为每一组XDR扫描对生成唯一标识符，并将该标识符保存至两张扫描图像文件中。特征提取（Feature Extraction，FE）9.5软件在提取数据时，会通过XDR标识符自动将两组扫描数据进行关联。经所有FE处理步骤后保留的信号即为经乘性去趋势、背景扣除后的处理信号（ProcessedSignal）。