Soluble plantain NSP inhibits adhesion of S. Typhimurium to the porcine B1OXI enterocyte cell-line in vitro.
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https://figshare.com/articles/dataset/_Soluble_plantain_NSP_inhibits_adhesion_of_S_Typhimurium_to_the_porcine_B1OXI_enterocyte_cell_line_in_vitro_/926427
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Pre-treatment (30 min) with soluble plantain NSP, dose-dependently blocked adhesion of (A) S. Typhimurium LT2 and (B) S. Typhimurium 4/74 to B1OXI cells (N = 3 experiments, each with n = 4 replicates); * PPPC) S. Typhimurium LT2 and (D) S. Typhimurium 4/74 into B1OXI cells was also blocked by plantain NSP. Data (mean ± SEM) expressed relative to adherence (or invasion) of vehicle-treated control (100%). Light microscopy of Giemsa-stained B1OXI cells and S. Typhimurium 4/74 in absence (E) or presence (F) of 10 mg/mL soluble plantain NSP. Arrows indicate bacteria.
经可溶性车前草非淀粉多糖(soluble plantain NSP)预处理30分钟后,可剂量依赖性阻断(A)鼠伤寒沙门氏菌(*Salmonella Typhimurium*)LT2株与(B)鼠伤寒沙门氏菌4/74株对B1OXI细胞的黏附能力;本实验共重复3次,每组设置4个复孔。*(原文此处疑似排版笔误,推测应为标注(C))* 鼠伤寒沙门氏菌LT2株与(D)鼠伤寒沙门氏菌4/74株对B1OXI细胞的侵袭过程,同样可被车前草NSP阻断。所有数据以均值±标准误(mean ± SEM)表示,以溶剂处理对照组的黏附(或侵袭)水平为100%进行归一化。光学显微镜观察结果展示了未添加(E组)与添加(F组)10 mg/mL可溶性车前草NSP时,吉姆萨染色的B1OXI细胞与鼠伤寒沙门氏菌4/74的形态,箭头指示细菌所在位置。
创建时间:
2016-02-23



