Altered pattern of circulating miRNAs in HIV lipodystrophy perturbs key adipose differentiation and inflammation pathways. Altered pattern of circulating miRNAs in HIV lipodystrophy perturbs key adipose differentiation and inflammation pathways

We identified a microRNA (miRNA) profile characterizing HIV lipodystrophy and explored the downstream mechanistic implications with respect to adipocyte biology and the associated clinical phenotype. Human preadipocytes transfected with mimic miR-20a-3p, anti–miR-324-5p, or anti–miR-186 induced consistent changes in latent transforming growth factor beta binding protein 2 (Ltbp2), Wisp2, and Nebl expression. Overall design: Human preadipocytes were transfected with miR-20a-3p mimic, anti–miR-186, anti–miR-324-5p,or control nontargeting miRNA and then cultured in differentiation medium for 12 additional days to give rise to mature adipocytes, after which RNA was collected and subjected to RNA-Seq.

本研究鉴定得到表征HIV相关脂肪代谢障碍（HIV lipodystrophy）的微小RNA（microRNA, miRNA）表达谱，并围绕脂肪细胞生物学及相关临床表型，探讨了其下游调控机制。将人前体脂肪细胞分别转染miR-20a-3p模拟物、抗miR-324-5p寡核苷酸或抗miR-186寡核苷酸后，可使潜伏转化生长因子β结合蛋白2（latent transforming growth factor beta binding protein 2, Ltbp2）、Wisp2及Nebl的表达产生一致性变化。实验设计概述：将人前体脂肪细胞分别转染miR-20a-3p模拟物、抗miR-186寡核苷酸、抗miR-324-5p寡核苷酸，或对照非靶向微小RNA，随后置于分化培养基中继续培养12天以诱导成熟脂肪细胞生成，之后收集RNA并进行RNA测序（RNA-Seq）。