Pollen forage of honey bees Targeted loci environmental

In light of recent pollinator declines and potential loss of pollination services, the has been increased basic and applied research on pollinator systems. Sequencing of phylogenetically informative “barcode” genetic loci has emerged over the past two decades as a robust tool for taxonomic classification that often achieves higher resolution than morphological or other classification methods. More recently, high-throughput sequencing has allowed “meta-barcoding” analysis of diverse mixtures, and this approach has begun to be applied to bee-collected. Here we develop a sequencing approach that differs from other approaches in the sequence that was analyzed, the use of a higher-capacity platform with more flexible multiplexing, and/or the development of a workflow for non-overlapping read pairs.鉴于近期传粉者种群衰减及传粉服务潜在丧失的现状，针对传粉者系统的基础与应用研究已显著增多。在过去二十年间，携带系统发育信息的“基因条形码（barcode）”基因位点测序技术已发展为一种可靠的分类学鉴定工具，其分辨率通常优于形态学或其他分类方法。近年来，高通量测序技术已可实现对多种混合样本的“元条形码（meta-barcoding）”分析，该方法已开始应用于蜜蜂采集的样本。本研究开发了一种新型测序方案，该方案在分析序列选用、采用复用灵活性更高的高容量测序平台，以及开发非重叠读对分析流程等方面，均与现有方法存在差异。