RNA-seq analysis of gene expression in male and female mouse liver

Gene expression in adult male and female mouse liver was assayed by RNA-seq, as part of a study on chromatin states in male and female mouse and their role in sex-biased liver gene expression (A Sugathan and DJ Waxman (2013) Mol Cell Biol. 33:3594-3610. doi: 10.1128/MCB.00280-13). Overall design: Total liver RNA was prepared from 12 individual male and 12 individual female mice. Four RNA pools, comprised of RNA isolated from 6 individual male or female livers (2 pooled biological replicates for each sex) were then prepared and used for RNA-seq.

本数据集通过RNA测序（RNA-seq）检测成年雌雄小鼠肝脏的基因表达情况，该研究属于一项探究雌雄小鼠染色质状态及其在性别偏好性肝脏基因表达中作用的课题（A Sugathan、DJ Waxman，2013，《分子与细胞生物学》（Mol Cell Biol.），33卷：3594-3610，DOI: 10.1128/MCB.00280-13）。整体实验设计：从12只雄性和12只雌性小鼠中分别提取总肝脏RNA。随后将每6只同性别的个体小鼠的肝脏RNA混合，制备得到4个RNA混合样本（每个性别设置2个混合生物学重复），并将这些样本用于RNA-seq测序。