Global proteome of Atg16L1flox:CAG-CreTmx WT and KO neurons

The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient primary neurons. Therefore, cortico-hippocampal neurons were isolated from Atg16L1flox:CAG-CreTmx mice and treated with Tamoxifen (Tmx) to induce the knockout (KO) or EtOH for wildtype (WT) in-vitro. WT and KO Neurons were harvested at day in-vitro (DIV) 15-16, and global proteome analysis was measured by LC-MS/MS.

本实验旨在鉴定野生型及自噬（autophagy）缺陷的原代神经元（primary neurons）中的自噬靶点。为此，我们从Atg16L1flox:CAG-CreTmx小鼠中分离皮层海马神经元（cortico-hippocampal neurons），并将其分为两组：一组用他莫昔芬（Tamoxifen, Tmx）处理以诱导敲除（KO）模型，另一组用乙醇（EtOH）处理以获得野生型（WT）体外神经元。随后于体外培养第15-16天（days in vitro, DIV）收集两组神经元，并通过液相色谱-串联质谱（Liquid Chromatography-Tandem Mass Spectrometry, LC-MS/MS）开展全局蛋白质组分析。