Epigenetic transcriptional reprogramming by WT1 mediates a repair response during podocyte injury [ChIP-seq]. Epigenetic transcriptional reprogramming by WT1 mediates a repair response during podocyte injury [ChIP-seq]

We profiled Wt1, a key transcription factor, in purified murine podocytes based on ChIP-seq during the time course of podocyte injury by ADR. Overall design: Genomic binding/occupancy profiling of Wt1 during podocyte injury by high throughput sequencing

本研究依托染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing，ChIP-seq）技术，在纯化的小鼠足细胞（murine podocytes）中，针对阿霉素（Adriamycin，ADR）诱导的足细胞损伤时间进程，对关键转录因子Wt1进行了基因组结合谱分析。实验设计概述：通过高通量测序技术，对阿霉素诱导的足细胞损伤过程中Wt1的基因组结合与占据情况开展谱图分析。