Whole genome sequencing (WGS) of Plasmodium yoelii YM. Whole genome sequencing (WGS) of Plasmodium yoelii YM

Dr Tony Holder's laboratory (NIMR, London) has been successful in deleting one of the RH family genes (Py01365) by transfection and insertion of the TgDHFR gene, and cloned the resulting parasite in YM background. The gene expression patterns of the mutant parasite line were compared to that of the wild type YM parasite. Since the work was done in YM background and the currently available reference genome of P. yoelii was obtained from the 17XNL strain, we had to map all the RNA-seq Illumina reads onto the genome of the 17XNL strain. There are significant phenotypic differences between the 17XNL and the YM strain. Hence, we wanted to obtain about >100X Illumina coverage on the genome sequence of the YM strain so that all the genomic differences between the YM and 17XNL strains could be taken into account while comparing the expression profiles. The sequencing of the P. yoelii YM strain allowed us to come up with a catalogue of genomic differences between these two phenotypically distinct P. yoelii strains.. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

伦敦国家医学研究所（NIMR）的Tony Holder博士实验室通过转染并插入TgDHFR基因，成功敲除了RH家族基因之一（Py01365），并在YM遗传背景下克隆获得了对应的突变疟原虫株。本研究将该突变疟原虫株的基因表达模式与野生型YM疟原虫株进行了对比。由于实验基于YM遗传背景开展，而目前公开的约氏疟原虫（P. yoelii）参考基因组来源于17XNL株，因此我们需将所有Illumina RNA-seq测序读段比对至17XNL株的基因组序列。

17XNL与YM株存在显著的表型差异，因此我们计划对YM株的基因组序列进行≥100倍的Illumina测序覆盖度，以便在比较基因表达谱时，充分考量YM与17XNL株之间的所有基因组差异。对约氏疟原虫YM株的测序工作，帮助我们构建了这两株表型迥异的约氏疟原虫株的基因组差异目录。

本数据集属于预出版发布数据。若需了解威康信托桑格研究所（Wellcome Trust Sanger Institute）共享的预出版数据的规范使用方法（包括任何出版延期相关细节），请访问http://www.sanger.ac.uk/datasharing/