小鼠心梗模型中长时程移植细胞追踪数据集

为追踪心梗后移植细胞，利用长时程追踪细胞增殖的工具鼠，我们首先检测了稳态下心脏中心肌细胞的增殖。长时程追踪细胞增殖的工具鼠是 Ki67-CrexER(Ki67-Cre-rox-ER-rox)，显示 Ki67 活动的报告基因小鼠是 R26-GFP。为了能够特异在心肌细胞中开启细胞增殖示踪，我们引入了特异靶向心肌细胞的腺相关病毒 AAV9-cTNT-Dre。在心肌细胞中，Dre 识别 CrexER 中的 rox 位点，同源重组后将 ER 重组掉，Ki67-CrexER 转变为 Ki67-Cre，心肌细胞发生增殖活动后 Ki67 启动子活跃启动 Cre 的表达，利用 R26-GFP 标记增殖的心肌细胞。

To track transplanted cells following myocardial infarction, we first detected cardiomyocyte proliferation in the heart under steady-state conditions using a tool mouse engineered for long-term cell proliferation tracing. The tool mouse for long-term cell proliferation tracing is Ki67-CrexER (Ki67-Cre-rox-ER-rox), and the reporter gene mouse that visualizes Ki67 activity is R26-GFP. To specifically initiate cell proliferation tracing in cardiomyocytes, we introduced the cardiomyocyte-targeting adeno-associated virus AAV9-cTNT-Dre. In cardiomyocytes, Dre recognizes the rox sites within CrexER; after homologous recombination, the ER segment is excised, converting Ki67-CrexER to Ki67-Cre. When cardiomyocytes undergo proliferation, the Ki67 promoter is activated to drive Cre expression, and R26-GFP is used to label proliferating cardiomyocytes.