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在本研究中，我们采用 HT22 细胞系作为实验模型来研究氧化应激对其基因表达谱的影响。源自小鼠海马神经元的 HT22 细胞系经常用于神经生物学研究，特别是有关神经退行性疾病和氧化应激机制的研究。采用特定方法诱导氧化应激，我们成功地在 HT22 细胞中建立了氧化应激模型。建立该模型后，我们采用高通量测序技术对氧化应激条件下的 HT22 细胞转录组进行了全面分析。本研究的目的有两个：首先，鉴定与氧化应激反应相关的差异表达基因，其次，进一步分析这些基因的功能富集。进行该分析的目的是揭示氧化应激对神经元细胞基因表达的调控机制。

In this study, we employed the HT22 cell line as an experimental model to investigate the effects of oxidative stress on its gene expression profile. The HT22 cell line, derived from mouse hippocampal neurons, is widely utilized in neurobiological research, especially studies focused on neurodegenerative diseases and the mechanisms underlying oxidative stress. By inducing oxidative stress through specific approaches, we successfully established an oxidative stress model in HT22 cells. Following the establishment of this model, we conducted a comprehensive transcriptomic analysis of HT22 cells under oxidative stress conditions using high-throughput sequencing technology. The objectives of this study are twofold: first, to identify differentially expressed genes (DEGs) associated with oxidative stress responses; second, to perform further functional enrichment analysis of these genes. The aim of this analysis is to unravel the regulatory mechanisms through which oxidative stress modulates gene expression in neuronal cells.