Montipora capitata spawning gene expression Raw MiSeq Reads

This project examines gene expression during the normal spawning timing of corals that did and did not spawn. Samples were collected from 3 replicate Montipora capitata colonies for each condition at 18:00, 20:00 and 00:00 the night of 6/6/16. RNA extracted from samples was prepped using Illumina TruSeq Sample Prep v2 Guide and sequenced 2x150bp on the Illumina MiSeq.

本研究旨在探究正常产卵时序下，已产卵与未产卵珊瑚的基因表达特征。本研究于2016年6月6日夜的18:00、20:00及00:00三个时间点，针对两种实验条件分别采集3个生物学重复的鹿角杯形珊瑚（Montipora capitata）群体样本。从样本中提取的RNA按照Illumina TruSeq样本制备指南v2完成文库制备，并在Illumina MiSeq测序平台上开展2×150bp双端测序。