Gene expression profiling of whole testes in embryonic (gestational day 11, 12, 14, 16 & 18) and postnatal day 2 mice. (GUDMAP Series ID: 3)

The overall objective of this proposal is to map the temporal and spatial dynamics of gene expression in the fetal mouse testis at key developmental timepoints. Urogenital tract malformations are the most common birth defects in males and their incidence together with other male reproductive health concerns such as reduced fertility and testicular cancer are reportedly on the rise in the human population. To better understand the impact of genetic factors and environmental influences on testicular development, it is important to first understand normal gene expression patterns and signaling cascades within the fetal testis during development. The goal of this study is to identify cell-specific genes that can be used as biomarkers for key differentiation events. Keywords: developmental time course Day to day comparison in whole testes throughout development with triplicates at each time point. Each sample is a pair of testes from a single animal and each animal is taken from a different dam.

本项目的整体目标为解析关键发育时间节点下，胎鼠睾丸内基因表达的时空动态变化特征。泌尿生殖道畸形是男性最常见的出生缺陷，据报道，其发病率与男性生育力下降、睾丸癌等其他男性生殖健康问题一同在人群中呈上升趋势。为更好地解析遗传因素与环境影响对睾丸发育的作用机制，首先明确发育过程中胎鼠睾丸内的正常基因表达模式与信号通路级联反应至关重要。本研究的目标为鉴定可作为关键分化事件生物标志物的细胞特异性基因。关键词：发育时间进程；发育全程全睾丸组织的每日比对，且每个时间点设置三次生物学重复。每份样本取自单只动物的一对睾丸，且每只实验动物均来自不同的母鼠。