Expression data from BPH resistant and susceptible rice varieties

Bph6 is a gene that confers rice high resistance to its devastating pest BPH. Understanding the molecular responses of the resistant and susceptible varieties would pave the way to controlling the pest more effectively. To better understand the molecular mechanism underlying resistance to BPH mediated by Bph6, we conducted a genome-wide microarray analysis of NIL-BPH9 and 9311 at the early stage of BPH infestation. Leaf shealth of resistant and susceptible rice plants were collected at 0(without BPH infestation),6, 48h by BPH infestation for RNA extraction and hybridization on Affymetrix microarrays.

Bph6是一种可赋予水稻对其毁灭性害虫褐飞虱（BPH）高抗性的基因。解析抗性与易感水稻品种的分子响应机制，可为更高效地防治该害虫奠定理论基础。为深入解析由Bph6介导的抗褐飞虱分子机制，我们对褐飞虱侵染早期的NIL-BPH9与9311开展了全基因组芯片分析。分别于未受褐飞虱侵染的0小时，以及褐飞虱侵染后6小时、48小时采集抗性与易感水稻植株的叶鞘，用于RNA提取并在Affymetrix芯片上进行杂交。