CD4+T cells activation

Aanlysis of human resting CD4+T cells and cells activated by two different methods: CD4+ T cells unstimulated (be susceptible but not support to HIV-1) and stimulated either by CD3/CD28 costimulation (reversed susceptibility and resisted to HIV-1) or by PHA/IL-2 for six days (be susceptible and support to HIV-1) to investigate potential mechanism of reversing susceptible to HIV-1. We measured gene expression in resting human CD4+T cells and cells activated by two methods for six days which could induce different effects to HIV-1. Three independent experiments were performed at each treatments using different donors for each experiment.

本研究针对人类静息CD4+T细胞（resting CD4+T cells）以及两种不同方法活化的CD4+T细胞展开分析：未受刺激的CD4+T细胞（易感染HIV-1但无法支持其复制）、经CD3/CD28共刺激活化的CD4+T细胞（易感性逆转并抵抗HIV-1感染），以及经PHA/IL-2刺激培养6天的CD4+T细胞（易感染HIV-1且支持其复制），旨在探究逆转HIV-1易感性的潜在分子机制。我们对静息人类CD4+T细胞，以及经两种可诱导不同HIV-1感染效应的方法活化培养6天的细胞进行了基因表达检测。每个处理组均设置三次独立实验，每次实验使用不同的健康供者样本。