Sirtuin 1 Levels in Recurrent Implantation Failure

Abstract Sirtuin 1 has an important role in cellular processes, including apoptosis and cellular stress. The purpose of this study was to assess serum sirtuin 1 levels in women with recurrent implantation failure (RIF). In this cross-sectional study, we included 28 women with RIF, 29 healthy women who had conceived by in vitro fertilization (IVF), and 30 women with a 1-cycle failure of IVF as controls. Human serum nicotinamide adenine dinucleotide (NAD)-dependent deacetylase sirtuin-1 (SIRT1/SIRT2L1) levels were detected using a commercial colorimetric kit. Recurrent implantation failure patients have higher sirtuin 1 levels than non-pregnant women and healthy pregnant women, but this difference did not reach statistical significance due to the low number of patients in our study. These higher sirtuin 1 levels may result from the inflammation imbalance of RIF patients. The only statistically significant correlation found was between age and sirtuin (r = 0.277, p = 0.009).

摘要 沉默信息调节因子1（Sirtuin 1）在细胞凋亡、细胞应激等细胞进程中发挥重要作用。本研究旨在探讨复发性植入失败（recurrent implantation failure, RIF）女性的血清SIRT1水平。本横断面研究纳入28例RIF患者、29例经体外受精（in vitro fertilization, IVF）成功妊娠的健康女性，以及30例单次IVF周期失败的女性作为对照。采用商用比色试剂盒检测人血清烟酰胺腺嘌呤二核苷酸（nicotinamide adenine dinucleotide, NAD）依赖性脱乙酰酶SIRT1（又称SIRT2L1）的水平。结果显示，RIF患者的SIRT1水平高于未妊娠女性及健康妊娠女性，但由于本研究样本量较小，该差异未达到统计学显著性。RIF患者血清SIRT1水平升高可能与患者的炎症失衡有关。本研究仅发现年龄与SIRT1水平存在显著统计学相关性（r=0.277，p=0.009）。