Phenotypic impact of Toxoplasma gene knockout in wild-type vs. Δgra17 parasites.
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This table contains the phenotype scores for each Toxoplasma gene when knocked out in either wild-type (passage 1, 3, 4 and 8) or Δgra17 parasites (passage 1, 3, and 4). It further provides P-values that compare presence of sgRNAs at different passages in RH vs. Δgra17 parasites. Moreover, this table contains the FPKM (Fragments Per Kilobase of transcript per Million mapped reads) values derived from RNAseq data for the following strains: RHΔku80Δhxgprt, RHΔku80Δgra17, RHΔku80Δgra17+GRA17[low expression], and RH GRA17overexpression. (XLSX)
本表格包含了每个弓形虫(Toxoplasma)基因分别在野生型(传代1、3、4及8代)或Δgra17寄生虫(传代1、3及4代)中被敲除后的表型评分。此外,本表格还提供了P值,用于对比不同传代次数下RH与Δgra17寄生虫中单向导RNA(single guide RNA,sgRNA)的存在情况。同时,本表格还收录了来自RNA测序(RNAseq)数据的FPKM(每百万比对reads的转录本每千碱基片段数,Fragments Per Kilobase of transcript per Million mapped reads)值,涉及以下菌株:RHΔku80Δhxgprt、RHΔku80Δgra17、RHΔku80Δgra17+GRA17[低表达]以及RH GRA17过表达菌株。(XLSX)



