Identifying crossover locations in Arabidopsis thaliana sni1 Col-0 x Ct-1 F2 populations using genotyping-by-sequencing

Genomic DNA from 241 sni1-/- Col x Ct F2 individuals was extracted by CTAB and used to generate sequencing libraries as previously described (Hennig et al, 2018 G3). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3; Yelina et al, 2015 Genes & Dev).

采用十六烷基三甲基溴化铵（CTAB）法提取241株sni1-/- Col × Ct杂交F2个体的基因组DNA，并依照此前报道的方法（Hennig等，2018，《G3》）构建测序文库。测序数据借助TIGER分析流程，依照此前报道的方法（Rowan等，2015，《G3》；Yelina等，2015，Genes & Dev）完成交叉重组位点的鉴定。