High-throughput sequencing of small RNA during metamorphosis in the Japanese flounder (Paralichthys olivaceus). Paralichthys olivaceus

Many known miRNAs in fish come from zebrafish and fugu whose genome sequence data are available. The Japanese flounder undergoes typical metamorphosis which is characterized by major morphological, functional, and behavioral changes during growth due to this metamorphosis from larva to juvenile. Metamorphosis is a biological process by which an animal physically develops after birth or hatching, involving a conspicuous and relatively abrupt change in the animal's body structure through cell growth and differentiation. Here, the high-throughput sequencing was adopted to identify the miRNAs during metamorphosis in the Japanese flounder. We found abundant microRNAs during metamorphosis in the Japanese flounder. Overall design: Small RNAs were sequenced from metamorphosis stages of Japanese flounder

目前已报道的鱼类微小RNA（miRNAs）中，多数源自基因组序列数据已公开的斑马鱼（zebrafish）与河豚（fugu）。牙鲆（Japanese flounder）会经历典型的变态发育过程，该过程以形态、功能及行为层面的显著变化为特征——其生长过程中需完成从仔鱼到稚鱼的发育转变。变态发育是动物在出生或孵化后开展的生理性发育过程，通过细胞增殖与分化，使动物躯体结构发生显著且相对骤然的改变。本研究采用高通量测序技术，对牙鲆变态发育阶段的微小RNA进行鉴定，结果发现牙鲆变态发育阶段存在大量微小RNA。实验设计概述：对牙鲆变态发育各阶段的小型RNA进行测序。