Transcriptome of mature erythrocytes

Purpose: This study identifies the top transcripts expressed in mature erythrocytes and the top transcripts expressed in the polysome fractions of mature erythrocyte lysate Methods: RNA from total erythrocyte lysate and polysome fractions was isolated and purified using GeneJet RNA purification column (Thermo Fisher Scientific). The RNA library was prepared using NEBNext ULTRA II RNA library preparation kit for Illumina. The libraries were sequenced using Illumina Seq. Results: The transcriptome analysis revealed the abundance of mRNAs encoding globins genes, long non-coding RNAs, and micro RNAs. Deciphering the role and functions of these non-coding RNAs in these specialised cells could enable us to understand their biology in greater detail RNA transcriptome profiling was done for total mature erythrocyte lysates and polysome fractions using Illumina Seq

研究目的：本研究旨在鉴定成熟红细胞中高表达的转录本，以及成熟红细胞裂解液多聚体组分中高表达的转录本。
实验方法：采用GeneJet RNA纯化柱（Thermo Fisher Scientific，赛默飞世尔科技）分离纯化总红细胞裂解液及多聚体组分中的RNA；使用适配Illumina测序平台的NEBNext ULTRA II RNA文库制备试剂盒构建RNA文库，并通过Illumina测序（Illumina Seq）完成文库测序。
实验结果：转录组分析显示，编码珠蛋白基因的mRNA、长链非编码RNA（long non-coding RNAs）以及微小RNA（micro RNAs）呈现高丰度表达。解析这类特化细胞中这些非编码RNA的作用与功能，有助于更深入地阐明其生物学特性。本研究针对成熟红细胞总裂解液及多聚体组分开展了RNA转录组谱分析，测序采用Illumina Seq。