Electrical and synaptic integration of glioma into neural circuits

We report single cell RNA-seq data from patient-derived xenografts that were dissociated, FACS sorted into 96-well plates and profiled by Smart-seq2 and sequencing on an Illumina NextSeq500 Individual cells from two DIPG cell cultures derived by the Monje lab (DIPG VI and DIPG XIII-FL) were isolated by enzymatic and mechanical digestion followed by FACS sorting. cDNA libraries were generated using Smart-seq2 and Nextera XT with dual unique indexes. Libraries were sequenced on an Illumina NextSeq500, reads aligned by Bowtie and quantified by RSEM and alayses performed using Seurat.

本研究报道了来自患者来源异种移植瘤（patient-derived xenografts）的单细胞RNA测序（single cell RNA-seq）数据。该类样本经解离后，通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）分入选96孔板，采用Smart-seq2技术完成表达谱分析，并在Illumina NextSeq500测序平台上完成测序。本研究中，由Monje实验室构建的两株弥漫内生型桥脑胶质瘤（Diffuse Intrinsic Pontine Glioma, DIPG）细胞系（DIPG VI与DIPG XIII-FL）的单个细胞，通过酶解联合机械解离的方法分离，随后经FACS分选获得。实验采用Smart-seq2与带有双重唯一索引的Nextera XT试剂盒构建cDNA文库（complementary DNA library）。所获文库在Illumina NextSeq500平台上进行测序，测序reads经Bowtie完成序列比对、经RSEM完成基因定量，并通过Seurat软件开展后续数据分析。