RNA microarray analysis of human placenta in normal pregnancy and preeclampsia. RNA microarray analysis of human placenta in normal pregnancy and preeclampsia

To investigate the differentially expressed lncRNAs and mRNAs in human placenta between normal pregnancy and preeclampsia, we performed the human LncRNA microarray analysis of 8 samples from clinical patients. Overall design: Thirty preeclampsia patients (mean age of 33.20±0.46 years) and thirty control patients (mean age of 32.60±0.47 years) who received a caesarean section were included in this study. Preeclampsia was clinically diagnosed before caesarean. The main criteria for PE diagnosis were: SBP ≥ 140 or DBP ≥ 90 on two occasions at least 4 h apart; and proteinuria >0.3 g/24h. The control pregnancy was defined as: no medical complications and proteinuria, maternal blood pressure < 140/90 mm Hg. Of the thirty pairs of placenta samples, four pairs were randomly selected for lncRNA and mRNA microarray analysis, and the other samples were used for the following verification.

为探究正常妊娠与子痫前期（preeclampsia, PE）患者胎盘组织中差异表达的长链非编码RNA（lncRNA）与信使RNA（mRNA），本研究对8份临床患者样本进行了人源lncRNA芯片分析。
总体实验设计：本研究共纳入30例子痫前期患者（平均年龄33.20±0.46岁）与30例接受剖宫产的对照孕妇（平均年龄32.60±0.47岁）。子痫前期已于剖宫产术前经临床确诊，其主要诊断标准为：间隔至少4小时的两次测量中，收缩压（Systolic Blood Pressure, SBP）≥140mmHg或舒张压（Diastolic Blood Pressure, DBP）≥90mmHg，且24小时尿蛋白定量＞0.3g。对照妊娠的定义为：无内科并发症与蛋白尿，产妇血压＜140/90mmHg。在30组胎盘配对样本中，随机选取4组用于lncRNA与mRNA芯片分析，剩余样本用于后续验证实验。