RNA-seq analysis of Fusarium graminearum Z-3639 in heat stress

Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. To dissect cellular responses toward heat stress in the plant pathogenic fungus F. graminearum, we compared transcriptomes of the fungal cultures incubated in normal temperature condition (25 ºC) and in high temperature condition (37 ºC) for 15 min. Overall design: 6 samples examined: 24 h-old mycelia from complete medium (CM) of Fusarium graminearum wild-type Z-3639 were incubated in normal temperature condition (25 ºC) and in high temperature condition (37 ºC) for 15 min.

禾谷镰孢（Fusarium graminearum，有性态为玉蜀黍赤霉Gibberella zeae）是侵染小麦、大麦、玉米等主要谷类作物的重要病原真菌。为解析该植物病原真菌对热胁迫的细胞应答机制，我们对在正常温度（25℃）与高温（37℃）条件下培养15分钟的真菌培养物的转录组进行了比较分析。实验总体设计：共检测6个样本：将在完全培养基（complete medium，CM）上培养24小时的禾谷镰孢野生型菌株Z-3639的菌丝体，分别置于25℃正常温度与37℃高温条件下处理15分钟。