Gene expression profiles of splenic pDCs in C57Bl6/J and Siglec-H deficient mice. Gene expression profiles of splenic pDCs in C57Bl6/J and Siglec-H deficient mice

The steady-state gene expression of pDCs of two different mouse strains was analyzed. Splenic plasmacytoid dendritic cells (pDCs) of Siglec-H knock-out mice on C57Bl& background and the Siglec-H expression littermates (+/+) were enriched and sorted by FACS before generating RNA, followed by cDNA generation and sequencing. Overall design: There are 11 samples in total. Five replicate for the Siglec-H +/+ and six for the Siglec-H -/- condition.

本研究针对两种不同小鼠品系的浆细胞样树突状细胞（plasmacytoid dendritic cells, pDCs）的稳态基因表达特征展开分析。以C57Bl&背景下的Siglec-H基因敲除小鼠及其Siglec-H阳性同窝野生型小鼠（+/+）的脾脏pDCs为实验材料，通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS）完成细胞富集与分选，随后依次进行RNA提取、互补脱氧核糖核酸（complementary DNA, cDNA）合成及测序。实验整体设计：本研究共计11个样本，其中Siglec-H +/+组设置5个生物学重复，Siglec-H -/-组设置6个生物学重复。