Day-night rhythm in murine retinal pigment epithelium

Murine RPE transcriptomes were generated for 6 time points during a 24 hour period. Our data demonstrates that genes involved in ATP generation have a high expression at night whereas genes involved in β-oxidation and cholesterol synthesis show a high expression in the morning. Additionally, genes were identified in the glycerophospholipid metabolism pathway, suggesting the generation of glycerophospholipids at night. Finally, genes involved in intracellular trafficking with different expression patterns during the day-night cycle were identified and we identified genes involved in actin cytoskeleton building, remodelling and crosslinking with a high expression in the morning. Murine RPE transcriptomes were generated for 6 time points during a 24 hour period (ZT0, 2, 4, 9, 14, 19). In total 30 samples were sequenced because each time point was repeated 5 times (n=5).

本研究针对24小时周期内的6个时间点，构建了小鼠视网膜色素上皮（Retinal Pigment Epithelium, RPE）转录组。本研究数据表明，参与ATP生成的基因在夜间呈现高表达水平，而参与β-氧化与胆固醇合成的基因则于早晨高表达。此外，研究在甘油磷脂代谢通路中鉴定出相关基因，提示夜间存在甘油磷脂的合成过程。最后，本研究鉴定出参与细胞内运输且在昼夜周期中呈现差异化表达模式的基因；同时鉴定出参与肌动蛋白细胞骨架构建、重塑与交联的基因，此类基因于早晨呈现高表达水平。本研究针对24小时周期内的6个昼夜节律时间（Zeitgeber Time, ZT）点（ZT0、2、4、9、14、19）构建了小鼠RPE转录组。由于每个时间点设置5次生物学重复（n=5），总计共完成30例样本的测序。