Visium Spatial data of Brain section from Parkinson Mouse Model based on inducible expression of human a-syn constructs

Visium Spatial dataset of 20-months and 3-months old transgenic mice. The model demonstrates a-syn oligomer spread and accumulation at synapses within specific forebrain and midbrain regions, including the SNpc, enabling the observation of a-syn oligomer propagation by including a human a-syn protein complementation system expressing a-syn fused to non-fluorescent Venus YFP halves (V1S and SV2), which can reconstitute to fluorescent Venus YFP (V1S/SV2) when a-syn oligomerization takes place. This dataset includes 3 animals per condition with 3-4 slices per animal. Processed count matrices, metadata and objects with filtered, clustered, and annotated data can be found on Zenodo: 1. 10.5281/zenodo.15274014 2. 10.5281/zenodo.14988055

本数据集为针对20月龄与3月龄转基因小鼠的Visium空间转录组（Visium Spatial）数据集。该模型可展示α-突触核蛋白（α-syn）寡聚体在特定前脑与中脑区域（含黑质致密部（Substantia Nigra Pars Compacta, SNpc））的突触处的扩散与聚集；实验引入人源α-突触核蛋白蛋白互补系统，将α-突触核蛋白与非荧光的Venus黄色荧光蛋白（Venus YFP）的两个功能片段（V1S与SV2）分别融合表达，当α-突触核蛋白发生寡聚化时，两个片段可重组为具有荧光活性的Venus黄色荧光蛋白（V1S/SV2），进而实现对α-突触核蛋白寡聚体传播过程的直接观测。本数据集每组实验条件包含3只实验动物，每只动物对应3-4张脑组织切片。已完成过滤、聚类与注释的处理后计数矩阵、元数据及对应数据对象均可在Zenodo平台获取：1. 10.5281/zenodo.15274014；2. 10.5281/zenodo.14988055