Altered expression of PBMC miRNA profile following the third dose of inactivated COVID-19 vaccine

COVID-19 vaccination is the most effective approach to prevent severe disease and death. Inactivated vaccines are the most accessible type of COVID-19 vaccines in the developing countries. Several studies, including work from our group, have demonstrated that the third (booster) dose of inactivated COVID-19 vaccine induces robust humoral and cellular immune responses. The aim of this study was to examine miRNA expression profile in participants received a homogenous third dose of the CoronaVac vaccine. The discovery and validation cohorts included 6 volunteers.Samples of peripheral blood mononuclear cells (PBMCs) were harvested from healthcare volunteers before and 1-2 weeks after the third dose of CoronaVac.

新冠疫苗接种是预防重症疾病与死亡的最有效手段。灭活疫苗是发展中国家最易获取的新冠疫苗类型。包括本团队研究在内的多项研究已证实，新冠灭活疫苗第三剂（加强针）可诱导强效的体液免疫与细胞免疫应答。本研究旨在探究接受同源第三剂CoronaVac疫苗接种的受试者体内的microRNA (miRNA) 表达谱。本研究的发现队列与验证队列共纳入6名志愿者。在接种第三剂CoronaVac疫苗前及接种后1至2周，采集医护志愿者的外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）样本。