JMJD2A_Q2541.S2

modENCODE_submission_3784 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line S2-DRSC; Antibody JMJD2A-KDM4A Q2541 (target is JMJD2A/KDM4A)

modENCODE_submission_3784 本提交源自Gary Karpen主导的modENCODE项目。如需查阅modENCODE项目完整列表，请访问http://www.genome.gov/26524648。项目目标：本研究旨在确定125种染色体蛋白质在黑腹果蝇（Drosophila melanogaster）全基因组中的定位位点。所研究的蛋白质参与DNA复制、基因表达、基因沉默及遗传等基础染色体生物学功能。本研究将采用基因组平铺芯片（genomic tiling arrays）开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验。初步将使用三种细胞系与两个胚胎时期的染色质进行定位分析，后续还会将部分蛋白质的分析拓展至另外四种动物组织/发育阶段。关于数据使用的条款与规范，请参阅http://www.genome.gov/27528022 及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。实验类型：ChIP-chip。生物样本来源：细胞系：S2-DRSC；组织：胚胎来源细胞系；发育阶段：晚期胚胎阶段；性别：雄性；重复次数：4次；实验因子：S2-DRSC细胞系；抗体：JMJD2A-KDM4A Q2541（靶标为JMJD2A/KDM4A）