Ecoli_Cojugation

These are donor E coli cells (ANB35) and recipient Ecoli cells (ANB24). These are the same . cells described in the Science paper (Direct visualization of horizontal gene transfer). The . cells were mixed on the grid and then plunge frozen and imaged with cryo-ET without CLEM. . Targets were imaged with cryo-ET randomly.

本数据集包含供体大肠杆菌（E. coli）细胞ANB35与受体大肠杆菌（E. coli）细胞ANB24。上述细胞与发表于《科学》（Science）期刊、题为《水平基因转移的直接可视化》的论文中所描述的细胞完全一致。将细胞置于电镜载网中混合后进行快速冷冻，并在未使用关联光学与电子显微镜术（CLEM）的情况下通过冷冻电子断层扫描（cryo-ET）完成成像。成像靶标通过冷冻电子断层扫描（cryo-ET）进行随机成像。