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Prophylactic Treatment by Tailocins Prevents Plant Infection

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DataCite Commons2021-06-21 更新2024-08-18 收录
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https://figshare.com/articles/dataset/Prophylactic_Treatment_by_Tailocins_Prevents_Plant_Infection/12814205/4
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Files containing a roadmap to creating the Receptor binding protein deletion mutant (DBL1424) as well as the in cis complement strain (DBL1701) and a Breseq output file showing genotypes for these strains compared to the USA011R genome. <br>Also included are .fasta files for each of the constructs, as well as unmodified primary pictures underlying figures in the manuscript.<br>I also include an overlay picture showing activity (or lack thereof) of tailocin preparations (supernatant and PEG prepared) against a suite Pseudomonas syringae pv. syringae B728a (aka DAB2). A white box indicates preparations from P. aeruginosa PAO1.<br>Lastly, I include an unaltered picture showing the retest of tailocin sensitivity from colonies of PsyB728a that grew on plants despite treatment with supernatants containing tailocins that work against this strain.

本数据集包含用于构建受体结合蛋白缺失突变株(DBL1424)以及顺式互补菌株(DBL1701)的技术路线文档,同时附带Breseq分析输出文件,该文件可展示上述菌株相较于USA011R基因组的基因型特征。 此外还收录了各分子构建体的.fasta格式文件,以及论文稿件中配图所用的未修改原始图像。 本数据集还包含一张叠加图像,用于展示尾素(tailocin)制剂(包括上清液制剂与聚乙二醇(PEG)制备制剂)对丁香假单胞菌丁香致病变种B728a(又名DAB2)的杀伤活性(或无活性状态)。图中白色方框标注的是铜绿假单胞菌(P. aeruginosa) PAO1的制剂。 最后,本数据集附带一张未编辑的图像,呈现了经靶向该菌株的尾素上清液处理后,仍可在植物上生长的PsyB728a菌落的尾素敏感性复测结果。
提供机构:
figshare
创建时间:
2021-06-21
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