Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 1 of 3: single-cell reporter measurements.

The nuclear-cytoplasmic pulsing behavior of FoxO3a fluorescent reporters (FoxO3aN400-Venus) with or without AKT- and ERK-dependent phosphorylation site mutations was assessed in two mammary epithelial cell lines at the single-cell level using live imaging of cells that were untreated or treated with one of five growth factors.

本研究在两种乳腺上皮细胞系中，于单细胞水平下，采用未经处理或经五种生长因子中任意一种处理的细胞活细胞成像技术，评估了带有或不带有AKT及ERK依赖性磷酸化位点突变的叉头框蛋白O3a（FoxO3a）荧光报告基因（FoxO3aN400-Venus）的核质脉冲行为。