Additional file 2 of Heat-responsive microRNAs participate in regulating the pollen fertility stability of CMS-D2 restorer line under high-temperature stress

Additional file 2: Table S1. Overview of reads from raw data to cleaned sequences in 12 small RNA sequencing (sRNA-seq) libraries. Table S2. Classification statistics of repetitive sequences in total and unique data of 12 sRNA-seq libraries. Table S3. Length distribution of counts of total and unique sRNA sequences in 12 sRNA-seq libraries. Table S4. Detailed summary of identified known and predicted miRNAs and their expression levels in this study. Table S5. Statistics of the number of pre-miRNAs and unique miRNAs in different categories for each sample. Table S6. Length distribution of all identified miRNAs. Table S7. MiFam-based family analysis of identified miRNAs. Table S8. Conservation of the identified miRNAs with other species. Table S9. Conservation profile of the identified miRNA family members. Table S10. The first base preference of mature miRNAs. Table S11. Base preference in different positions of mature miRNAs. Table S12. Identification and expression level analysis of differentially expressed miRNAs. Table S13. Identification and expression level analysis of differentially expressed genes. Table S14. GO functional enrichment analysis of all DEGs. Table S15. KEGG pathway enrichment analysis of all DEGs. Table S16. Overview of degradome sequencing data output for P_NH and P_SH. Table S17. Target gene and predicted cleavage site information validated through degradome sequencing analysis in P_NH and P_SH. Table S18. GO enrichment analysis of the miRNA targets. Table S19. KEGG enrichment analysis of the miRNA targets. Table S20. KEGG enrichment annotation analysis of miRNA targets with negative regulatory relationship under HT stress.

附加文件2：表S1：12个小RNA测序（small RNA sequencing, sRNA-seq）文库中从原始数据至清洁序列的reads概况。表S2：12个sRNA-seq文库总数据与唯一数据中的重复序列分类统计。表S3：12个sRNA-seq文库中总sRNA序列与唯一sRNA序列的计数长度分布。表S4：本研究中鉴定得到的已知及预测型microRNA (miRNA)及其表达水平的详细汇总。表S5：各样本不同类别下的前体miRNA (pre-miRNA)与唯一miRNA的数量统计。表S6：所有鉴定到的miRNA的长度分布。表S7：基于MiFam的鉴定miRNA家族分析。表S8：鉴定到的miRNA与其他物种的保守性分析。表S9：鉴定到的miRNA家族成员的保守性特征。表S10：成熟miRNA的首碱基偏好性。表S11：成熟miRNA不同位置的碱基偏好性。表S12：差异表达miRNA的鉴定与表达水平分析。表S13：差异表达基因的鉴定与表达水平分析。表S14：所有差异表达基因 (differentially expressed genes, DEGs)的GO功能富集分析。表S15：所有DEGs的KEGG通路富集分析。表S16：P_NH与P_SH的降解组测序数据产出概况。表S17：通过P_NH和P_SH的降解组测序分析验证的靶基因及预测剪切位点信息。表S18：miRNA靶基因的GO富集分析。表S19：miRNA靶基因的KEGG富集分析。表S20：高温胁迫 (HT stress)下具备负调控关系的miRNA靶基因的KEGG富集注释分析。