single-cell RNA-seq of multiple myeloma

Preprocessed single-cell RNA-sequencing data of multiple myeloma. Bone marrow mononuclear cells from a patient with multiple myeloma were purified from bone marrow aspirates by standard density gradient centrifugation using Lympholyte-H (Cedarlane Laboratories Ltd.) and were cryopreserved in CELLBANKER 1 (Takara Bio Inc.). Frozen cells from the multiple myeloma patient were resuspended in RPMI-1640 medium with 10% FBS. The cDNA library was constructed from the sample using the Chromium Single Cell 3′ Library & Gel Bead Kit v2 (10x Genomics) according to the manufacturer’s instructions. The cDNA library was sequenced on an Illumina HiSeq 2500 platform. Alignment to human genome (GRCh38), de-duplication, filtering and generation of gene expression matrixes were performed with the Cell Ranger v3.0.2 (10x Genomics) according to the manufacturer’s instructions.

本数据集为多发性骨髓瘤（multiple myeloma）的预处理单细胞RNA测序数据。研究人员从一名多发性骨髓瘤患者的骨髓穿刺液中，通过Lympholyte-H（Cedarlane Laboratories Ltd.）的标准密度梯度离心法纯化得到骨髓单个核细胞，并使用CELLBANKER 1（Takara Bio Inc.）进行冻存。将该患者的冻存细胞重悬于含10%胎牛血清（FBS）的RPMI-1640培养基中。使用Chromium Single Cell 3′ Library & Gel Bead Kit v2（10x Genomics）试剂盒，严格遵循厂商指导手册的操作流程，从该样本构建cDNA文库。将构建完成的cDNA文库在Illumina HiSeq 2500测序平台上进行测序。参照厂商指导手册，使用Cell Ranger v3.0.2（10x Genomics）软件完成序列比对至人类基因组GRCh38、去重、过滤以及基因表达矩阵生成的全部流程。