Genome-wide Definition of the SigF Regulon in Mycobacterium tuberculosis (Expression). Mycobacterium tuberculosis H37Rv

The genome wide transcriptome analysis to identify the M.tuberculosis sigma factor Rv3286c (SigF) dependent transcripts as well as Pristimaycin induced transcripts Overall design: Pristinamycin induced transcripts were identified by comparing the cDNA extracted from H37Rv::pMY769 with and without pristinamycin (3 biological replicates). SigF controlled transcripts were identified by comparing the cDNA extracted from H37Rv::pMYsigF with and without pristinamycin (4 biological replicates).

本研究通过全基因组转录组分析（genome-wide transcriptome analysis），旨在鉴定结核分枝杆菌（M. tuberculosis）西格玛因子Rv3286c（SigF）依赖的转录本，以及普那霉素（Pristinamycin）诱导的转录本。实验设计概述：普那霉素诱导的转录本通过对比添加与不添加普那霉素的H37Rv::pMY769菌株提取的cDNA进行鉴定，设置3次生物学重复；SigF调控的转录本则通过对比添加与不添加普那霉素的H37Rv::pMYsigF菌株提取的cDNA进行鉴定，设置4次生物学重复。