RNA-seq of transgenic Arabidopsis thaliana seedlings expressing the Pseudomonas syringae effector HopBB1

This study evaluates the transcriptome of transgenic Arabidopsis seedlings expressing the effector HopBB1 from the bacteria Pseudomonas syringae Arabidopsis thaliana seedlings were grown under short day conditions (9h of light at 21°C - 15h of dark at 18°C) for two weeks. Samples were harvested in the beginning of the light period and used for RNA extraction and Illumina sequencing. Lines Col-0 (3 replicates) and 35S::HopBB1-10 (3 replicates) were used in the experiment. Each replicate corresponds to approximately 30 seedlings grown in the same pot.

本研究对表达丁香假单胞菌（Pseudomonas syringae）效应蛋白HopBB1的转基因拟南芥（Arabidopsis thaliana）幼苗的转录组进行分析。拟南芥幼苗于短日照条件（21℃下光照9小时、18℃下黑暗15小时）中培养两周。于光照周期起始时采集样本，用于RNA提取及Illumina测序。本实验使用Col-0株系（3个生物学重复）与35S::HopBB1-10株系（3个生物学重复），每个重复对应同一栽培盆中生长的约30株幼苗。