Gut microbiota-derived gamma-aminobutyric acid from metformin treatment reduces hepatic ischemia/reperfusion injury by inhibiting ferroptosis

Hepatic ischemia/reperfusion injury (HIRI) is a common and inevitable factor leading to poor prognosis in various liver diseases, making the outcomes of current treatments in the clinic unsatisfactory. Metformin has been demonstrated to be beneficial in alleviating HIRI in recent studies, however, the underpinning mechanism remains unclear. In this study, we found metformin mitigates HIRI-induced ferroptosis through reshaped gut microbiota in mice, which was confirmed by the results of fecal microbiota transplantation treatment but showed the elimination of the beneficial effects when gut bacteria were depleted using antibiotics. Detailedly, through 16S rRNA and metagenomic sequencing, we identified that the metformin-reshaped microbiota was characterized by the increase of gamma-aminobutyric acid (GABA) producing bacteria. This increase was further confirmed by the elevation of GABA synthesis key enzymes, glutamic acid decarboxylase, and putrescine aminotransferase, in gut microbes of ..., , , # Gut microbiota-derived gamma-aminobutyric acid from metformin treatment reduces hepatic ischemia/reperfusion injury by inhibiting ferroptosis

## I. Dataset Overview

This dataset contains the original data used in the eLife article, primarily supporting research on hepatic ischemia-reperfusion injury. The dataset comprises various types of data files, including but not limited to text files, image files, and analysis code.

# II. File Structure and Naming Convention

Data files are organized into separate folders based on their location in the manuscript, such as \"figure 1 data\", \"figure 2 data\", etc.

## III. Data Content

Text Data: Contains experimental measurements, statistical results, etc. All the data are put in Excel documents named such as “original data (Figure 1 data)” in the “Figure 1 data” file, etc.

Image Data: Includes experimental images, photomicrographs, etc., stored in TIFF or JPG format.

Acronyms Meaning: ALT and AST refer to glutamic pyruvic transaminase and gl...

肝缺血再灌注损伤（Hepatic ischemia/reperfusion injury, HIRI）是多种肝脏疾病预后不良的常见且不可避免的诱因，致使当前临床治疗效果难尽人意。近年研究证实二甲双胍可缓解HIRI，但其核心作用机制仍未明确。本研究发现，二甲双胍可通过重塑小鼠肠道菌群减轻HIRI诱导的铁死亡（ferroptosis），该结论经粪便菌群移植实验得到验证，但当使用抗生素清除肠道菌群后，二甲双胍的保护作用便会消失。具体而言，通过16S rRNA测序与宏基因组测序，我们鉴定出二甲双胍重塑的菌群以产γ-氨基丁酸（gamma-aminobutyric acid, GABA）菌丰度升高为特征。该变化进一步通过肠道菌群中GABA合成关键酶——谷氨酸脱羧酶与腐胺氨基转移酶的表达上调得到证实。
# 二甲双胍干预后肠道菌群衍生的γ-氨基丁酸通过抑制铁死亡减轻肝缺血再灌注损伤

## 一、数据集概览
本数据集包含发表于eLife期刊的论文所用原始数据，主要支撑肝缺血再灌注损伤相关研究。数据集涵盖多种类型的数据文件，包括但不限于文本文件、图像文件及分析代码。

## 二、文件结构与命名规范
数据文件根据其在论文手稿中的对应位置归类至不同文件夹，例如“图1数据”“图2数据”等。

## 三、数据内容
### 文本数据
包含实验测量值、统计分析结果等。所有数据均存放于对应文件夹内的Excel文档中，如“图1数据”文件夹内的“原始数据（图1数据）”。

### 图像数据
包含实验图像、显微照片等，以TIFF或JPG格式存储。

### 缩写释义
ALT与AST分别代表丙氨酸氨基转移酶（glutamic pyruvic transaminase）和……