Dlx1/2-Dependent Expression of Meis2 Promotes Neuronal Fate Determination in the Mammalian Striatum [CUT-Tag-seq]. Dlx1/2-Dependent Expression of Meis2 Promotes Neuronal Fate Determination in the Mammalian Striatum [CUT-Tag-seq]

Purpose:To identity genomic loci where MEIS2 binds at E14.5 in the LGE Methords: The CUT&Tag assay was performed using E14.5 LGEs dissected from wild-type CD1 mice and a MEIS2 mouse polyclonal antibody as described previously. Results: We derived a set of 13992 loci based on the presence of significant enrichment in MEIS2 libraries and no enrichment in the negative control datasets Overall design: The CUT&Tag assay was performed using E14.5 LGEs dissected from wild-type CD1 mice and a MEIS2 mouse polyclonal antibody as described previously.

研究目的：鉴定小鼠胚胎第14.5天（E14.5）外侧神经节隆起（Lateral Ganglionic Eminence，LGE）中MEIS2结合的基因组位点。
实验方法：按照既往研究方案，使用从野生型CD1小鼠中解剖获取的E14.5外侧神经节隆起组织，结合MEIS2小鼠多克隆抗体，开展CUT&Tag实验。
实验结果：基于MEIS2文库存在显著富集、阴性对照数据集无富集的筛选标准，我们得到了包含13992个基因组位点的数据集。
实验整体设计：按照既往研究方案，使用从野生型CD1小鼠中解剖获取的E14.5外侧神经节隆起组织，结合MEIS2小鼠多克隆抗体，开展CUT&Tag实验。