Identifying and overcoming a mechanism of resistance to Wee1 kinase inhibitor AZD1775 in high grade serous ovarian cancer cells (ES-2)

Treatment of Human high grade serous ovarian cancer cell line, ES-2, with WEE1 kinase inhibitor AZD1775 Overall design: The experiment comprises 16 samples (MKG1-16) from ES-2 and ES-2 derived resistant clones: duplicate cultures of untreated ES-2 parent and ES-2 parent treated for 24 hours with 100nM AZD1775, triplicate cultures of four resistant clones maintained in AZD1775- two clones resistant to 1000nM AZD1775 and their progenitors resistant to 250nM AZD1775

WEE1激酶抑制剂AZD1775处理人高级别浆液性卵巢癌细胞系ES-2实验
实验设计概况：本实验包含16份编号为MKG1至MKG16的样本，所有样本均源自ES-2亲本细胞及其衍生的耐药克隆：具体包括未接受处理的ES-2亲本细胞的2份重复培养物，以及经100nM AZD1775处理24小时的ES-2亲本细胞的2份重复培养物；另有4种持续暴露于AZD1775环境中培养的耐药克隆的3份重复培养物，其中2种克隆对1000nM AZD1775产生耐药性，其祖代细胞对250nM AZD1775产生耐药性。