Comparative analysis from hESC from different developmental sources of derivation

Whole genome expression analysis reveals that single blastomeres from day-3 human embryos and blastocysts show unique gene signature, thus hESC derived from these two developmental sources might show differential gene expression profile. Comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. hESC derived from single blastomeres (VAL-10B and VAL-11B) and from ICM (VAL-5, -7, and -8) were compared by global gene expression

全基因组表达分析显示，来自第3天人类胚胎的单个卵裂球（blastomere）与囊胚（blastocyst）呈现独特的基因特征谱，因此从这两种发育来源分离得到的人类胚胎干细胞（human embryonic stem cell, hESC）可能存在差异基因表达谱。比较基因表达分析结果表明，源自卵裂球的hESC与源自内细胞团（inner cell mass, ICM）的hESC之间无显著差异，这提示多能性人类胚胎干细胞的形成涉及全新的发育进程。本研究通过全局基因表达分析，对源自单个卵裂球（VAL-10B与VAL-11B）以及内细胞团（VAL-5、VAL-7与VAL-8）的hESC进行了对比。