DataSheet1_A novel near-infrared fluorescent probe for rapid sensing of HClO in living cells and zebrafish.docx

Reactive oxygen species (ROS) are significant active species in living organisms, and their coordination maintains the function of organelles to resist the invasion of foreign substances. Hypochlorous acid (HClO) is not only an eventful signaling species but also a kind of ROS, which plays an irreplaceable role in the immune system. However, its abnormal levels can cause cell damage or even apoptosis, which in turn leads to the onset of a series of diseases such as inflammation, neurological diseases, and even cancer. Based on this, we designed a near-infrared fluorescent probe with a large Stokes shift for ultrafast response to HClO. Furthermore, the probe exhibits excellent sensitivity and selectivity toward HClO over other species. The probe was successfully applied to visualize endogenous and exogenous HClO in living cells and in zebrafish. This unique study is the key to providing a trustworthy tool for imaging based on the in vitro and in vivo imaging of endogenous HClO, which possesses great potential for the use in future studies of HClO-related biology and pathology.

活性氧（Reactive oxygen species, ROS）是生物体内重要的活性物质，其协同作用维持细胞器功能以抵御外来物质侵袭。次氯酸（Hypochlorous acid, HClO）不仅是一类关键信号分子，同时也属于ROS的一种，在免疫系统中发挥着不可替代的作用。然而，其水平异常会引发细胞损伤甚至凋亡，进而诱发炎症、神经系统疾病乃至癌症等一系列疾病。基于此，我们设计了一种具备大斯托克斯位移（Stokes shift）、可超快响应次氯酸的近红外荧光探针。此外，该探针对次氯酸展现出优异的灵敏度与相较于其他活性物种的高选择性。该探针已成功应用于活细胞与斑马鱼体内源性及外源性次氯酸的可视化成像。这项创新性研究为基于内源性次氯酸的体外与体内成像提供了可靠工具，其在未来次氯酸相关的生物学与病理学研究中具有巨大应用潜力。