Microarray analysis of gene expression from MDA-MB-231 cells. Homo sapiens

We used human Affymetrix microarrays to identify the up- or down-regulated gene expressions from MDA-MB-231 cells infected with control vector or Flag-SR-IkBa Keywords: microarray Overall design: The pattern of gene expression from MDA-MB-231 cells transduced with retroviruses were analysed by RNA extraction and hybridization on Affymetrix microarrays. From expression profiles, we identified NF-kB target genes, and uncovered a novel and specific role of a gene in osteolytic bone metastasis.我们采用人类Affymetrix微阵列（Affymetrix microarrays），从感染对照载体或Flag-SR-IkBa的MDA-MB-231细胞中筛选上调或下调的差异表达基因。 关键词：微阵列 整体实验设计：通过RNA提取与Affymetrix微阵列杂交，分析经逆转录病毒转导的MDA-MB-231细胞的基因表达模式。本研究通过表达谱鉴定出核因子κB（NF-κB）的靶基因，并揭示了某一基因在溶骨性骨转移中全新且特异性的作用。