A 3D molecular atlas of the chick embryonic heart. A 3D molecular atlas of the chick embryonic heart

We performed a detailed analysis of gene expression in the 2-day (HH12) embryonic chick heart. RNA-seq of 13 microdissected heart regions reveals regionalised expression of about 15,000 genes (Dataset 1). Of these, 131 genes that are differentially expressed (FPKM ≥20, fold change ≥1.1-4.0) within a region compared to the other 12 regions were studied by in situ hybridisation (bold text in Dataset 1) and used to generate a 3D molecular map of the heart at this stage of development.

我们对2日龄（HH12）鸡胚心脏开展了基因表达的详细分析。通过对13个经显微切割的心脏区域进行RNA测序（RNA-seq），我们检测到约15000个基因存在区域特异性表达（数据集1）。其中，针对131个在某一区域与其余12个区域相比存在差异表达（FPKM≥20，折叠变化≥1.1~4.0）的基因，我们通过原位杂交（in situ hybridisation）进行了研究（对应数据集1中的粗体文本），并利用这些基因构建了该发育阶段心脏的三维分子图谱。