Annotated Metagenome-Assembled Genomes (MAGs) from the Southern California Bight

This dataset contains metagenome-assembled genomes (MAGs) reconstructed from four environmental metagenomes collected from the Southern California Bight. A co-assembly was generated using MEGAHIT, and genome binning was performed using a combination of MetaBAT2, CONCOCT, and SemiBin2, followed by refinement and dereplication with MetaWRAP and dRep, respectively.All MAGs were annotated using Prokka. Two filtering strategies were applied to select high- and medium-quality MAGs:<b>Initial filter</b>: Completeness ≥50%, contamination ≤10%, resulting in <b>174 MAGs</b>.<b>Stricter filter</b>: Completeness ≥70%, contamination ≤5%, and N50 ≥10,000 bp, resulting in <b>74 high-quality MAGs</b>.This archive includes the annotated Prokka output directories for all 174 MAGs, each labeled with the prefix <code>SPOT_MAG_XXX</code>.These annotated MAGs can be used for downstream functional or phylogenetic analyses in marine microbiome research.

本数据集包含从南加州湾（Southern California Bight）采集的4份环境宏基因组重建得到的宏基因组组装基因组（metagenome-assembled genomes, MAGs）。研究团队使用MEGAHIT进行共组装，结合MetaBAT2、CONCOCT及SemiBin2开展基因组分箱，随后分别通过MetaWRAP和dRep完成基因组的精细化与去冗余操作。所有MAGs均通过Prokka进行注释。
本研究采用两种筛选策略以筛选高质量及中等质量的MAGs：<b>初始筛选</b>：完整度≥50%、污染率≤10%，最终得到<b>174个MAGs</b>；<b>严格筛选</b>：完整度≥70%、污染率≤5%且N50≥10000 bp，最终得到<b>74个高质量MAGs</b>。
本存档包含全部174个MAGs的Prokka注释输出目录，每个目录均以<code>SPOT_MAG_XXX</code>作为前缀命名。
这些注释后的MAGs可用于海洋微生物组研究中的下游功能或系统发育分析。