TCR phosphoproteome using DP thymocytes stimulated by H2-Kb MHC tetramers

Quantitative phosphoproteome with OT-I TAP-/- mice DP thymocytes stimulated by H2-Kb tetramers for 2 min. The quantitative data were calculated by the spike-in standard lysates from DPK cells cultured in the heavy media containing 15N2 13C6-lysine and 15N4 13C6-arginine.

本数据集为经H2-Kb四聚体（H2-Kb tetramer）刺激2分钟的OT-I TAP基因敲除（TAP-/-）小鼠双阳性胸腺细胞（double positive thymocytes, DP thymocytes）的定量磷酸化蛋白质组数据。其定量结果通过以培养于含15N2 13C6-赖氨酸与15N4 13C6-精氨酸的重同位素标记培养基中的DPK细胞所制备的加标标准裂解物进行计算获得。