Effect of LPA and COX inhibitors on the production of PGE2.

a:pUterine strips from rats pregnant on day 5 of gestation were incubated for 6 h with LPA 50 µM alone or in the presence of indomethacin 1 µM (a non selective COX-1 and COX-2 inhibitor) or NS-398 1 µM (a selective COX-2 inhibitor). In another set of experiments, the tissue was incubated for 6 h with indomethacin or NS-398 alone. The production of PGE2 was determined by radioimmunoassay.将妊娠第5天的大鼠子宫条带单独与50 μM溶血磷脂酸（LPA）孵育6小时，或联合1 μM吲哚美辛（indomethacin，一种非选择性环氧合酶-1（COX-1）与环氧合酶-2（COX-2）抑制剂）或1 μM选择性环氧合酶-2抑制剂NS-398共同孵育。另有一组实验中，将该组织单独以吲哚美辛或NS-398孵育6小时。采用放射免疫分析法（radioimmunoassay）测定前列腺素E2（PGE2）的生成量。