High temperature transcriptomes of mutants in HDA9, PIF4 and Col-0 wild type of young Arabidopsis seedlings. High temperature transcriptomes of mutants in HDA9, PIF4 and Col-0 wild type of young Arabidopsis seedlings

We determined the transcriptomes of hda9-1, pif4-2 and wild type Col-0 seedlings of 2 day old and 7 day old, at control and high temperature conditions (22oC vs 27oC), in short day (8h) photoperiod Overall design: Pooled seedlings, grown on sterile MS-agar plates, harvested at dawn of day 3 after initiation of germination (2 day old) and of day 8 (7 day old), in 3 replicates per mutant of 3 pooled independent batches (>50 seedlings per batch), genotype and temperature environment in short day conditions.

本研究对短日照（8小时）光周期条件下，2日龄与7日龄的hda9-1、pif4-2突变体及野生型Col-0幼苗，在对照温度（22℃）与高温（27℃）条件下的转录组（transcriptome）进行了测定。
实验设计概述：所有供试幼苗均培养于无菌MS-琼脂平板上，分别于萌发后第3天黎明（对应2日龄幼苗）及第8天黎明（对应7日龄幼苗）收获；每个基因型设置3次生物学重复，每次重复由3个独立的混合幼苗批次组成，每个批次包含至少50株幼苗，所有实验均在短日照条件下开展，涉及基因型与温度环境两类处理变量。