Transcriptome profiling of Bacillus subtilis OKB105 in response to rice seedlings

Plant growth-promoting rhizobacteria (PGPR) are soil beneficial microorganisms that colonize plant roots for nutritional purposes and accordingly benefit plants by increasing plant growth or reducing disease. But it still remains unclear which mechanisms or pathways are involved in the interactions between PGPR and plants. To understand the complex plant-PGPR interactions, the changes in the transcriptome of typical PGPR standard Bacillus subtilis in responding to rice seedlings were analyzed. We compared and anylyzed the transcriptome changes of the bacteria Bacillus subtilis OKB105 in response to rice seedings for 2 h. Total RNA was extracted and Random priming cDNA synthesis, cDNA fragmentation and terminal labeling with biotinylated GeneChip DNA labeling reagent, and hybridization to the Affymetrix GeneChip Bacillus subtilis Genome Array.

植物促生根际细菌（Plant growth-promoting rhizobacteria, PGPR）是一类定殖于植物根系以获取营养的土壤有益微生物，可通过促进植物生长或减轻植物病害使宿主植物获益。但目前学界仍未明确PGPR与植物互作所涉及的具体机制与信号通路。为解析植物与PGPR间的复杂互作机制，本研究针对典型PGPR模式菌株枯草芽孢杆菌（Bacillus subtilis）响应水稻幼苗的转录组变化展开分析。我们对枯草芽孢杆菌OKB105响应水稻幼苗培养2小时后的转录组变化进行了比较与分析。实验过程中先提取总RNA，采用随机引物法合成cDNA，随后进行cDNA片段化，使用生物素化GeneChip DNA标记试剂完成末端标记，再与Affymetrix GeneChip枯草芽孢杆菌基因组芯片进行杂交。