Dataset for: Everything you wanted to know about phase and reference frequency in one- and two-dimensional NMR Spectroscopy

The fundamental concept of phase is discussed in this tutorial aimed at providing students with an explanation of the delays and processing parameters they may find in NMR pulse programs. We consider the phase of radio-frequency pulses, receiver, magnetization and how all these parameters are related to phases and offsets of signals in spectra. The impact of the off-resonance effect on the phase of the magnetization is discussed before presenting an overview of how adjustment of the time reference of the free induction decay (FID) avoids first-order correction of the phase of spectra. The main objective of this tutorial is to show how the relative phase of a pulse and the receiver can be used to change the reference frequency along direct and indirect dimensions of NMR experiments. Unusual applications of phase incrementation with non-90 degree angles will be illustrated on 1D and 2D NMR spectra.

本教程以相位（phase）这一基础概念为核心展开，旨在为学生讲解核磁共振（NMR）脉冲程序中常见的延迟与处理参数。我们将探讨射频脉冲（radio-frequency pulses）、接收器（receiver）与磁化强度（magnetization）的相位，同时厘清上述各参数与谱图信号相位及偏移量之间的关联。在介绍如何通过调整自由感应衰减（free induction decay, FID）的时间基准以规避谱图相位的一阶校正之前，我们将先讨论偏共振效应（off-resonance effect）对磁化强度相位的影响。本教程的核心目标在于演示：如何利用脉冲与接收器的相对相位，对核磁共振实验的直接维度与间接维度上的参考频率进行调整。采用非90度角（non-90 degree angles）的相位增量（phase incrementation）的非常规应用案例，将通过一维（1D）与二维（2D）核磁共振谱图进行示例演示。