Genome-wide binding sites for Arabidopsis REPLUMLESS. Arabidopsis thaliana

The Arabidopsis thaliana REPLUMLESS gene (RPL), also known as PENNYWISE (PNY) and BELLRINGER (BLR) encodes a BEL1-like TALE homeodomain (BLH) transcription factor that controls multiple aspects of meristem and floral development, including meristem maintenance, phyllotaxis, transition to flowering, stem development and floral organ patterning [1-3]. As part of a screen for genes that mediate the function of RPL in the processes above, we performed ChIP-seq to identify genome-wide RPL binding sites within inflorescence apices. 1. Byrne, M.E., Groover, A.T., Fontana, J.R., and Martienssen, R.A. (2003). Development 130, 3941-3950. 2. Roeder, A.H.K., Ferrandiz, C., and Yanofsky, M.F. (2003). Current Biology 13, 1630-1635. 3. Smith, H.M.S., and Hake, S. (2003). Plant Cell 15, 1717-1727. Overall design: Plants expressing GFP-tagged RPL (pRPL:RPL-GFP) were used for ChIP-seq with anti-GFP antibodies; wild-type controls (Landsberg-erecta) were used as epitope-negatve controls.

拟南芥（Arabidopsis thaliana）的REPLUMLESS基因（RPL，又名PENNYWISE（PNY）与BELLRINGER（BLR））编码一类类BEL1的TALE同源域（BLH）转录因子，该因子可调控分生组织与花发育的多个进程，包括分生组织维持、叶序排列、开花转变、茎发育以及花器官模式建成[1-3]。为筛选介导RPL在上述进程中发挥功能的基因，我们通过染色质免疫共沉淀测序（ChIP-seq）技术，在花序顶端组织中鉴定全基因组范围的RPL结合位点。

参考文献：
1. Byrne, M.E.、Groover, A.T.、Fontana, J.R. 与 Martienssen, R.A.（2003）。《Development》，130卷，3941-3950页。
2. Roeder, A.H.K.、Ferrandiz, C. 与 Yanofsky, M.F.（2003）。《Current Biology》，13卷，1630-1635页。
3. Smith, H.M.S. 与 Hake, S.（2003）。《Plant Cell》，15卷，1717-1727页。

实验整体设计：采用携带GFP标签RPL（pRPL:RPL-GFP）的转基因植株，以抗GFP抗体开展染色质免疫共沉淀测序（ChIP-seq）实验；以野生型对照（兰兹伯格直立生态型，Landsberg-erecta）作为表位阴性对照。