Sinorhizobium meliloti as a model system to study Ca. Liberibacter asiaticus regulators. [RpoH]. Sinorhizobium meliloti as a model system to study Ca. Liberibacter asiaticus regulators. [RpoH]

We characterized transcriptomes of a Sinorhizobium meliloti rpoH1rpoH2 deletion mutant (RFF231; Lang et al. 2018, mSphere 3:e00454-18) expressing either Ca. Liberibacter asiaticus rpoH or Sinorhizobium meliloti rpoH1 Overall design: Gene expression profiling of Sinorhizobium meliloti strains was performed using custom Affymetrix GeneChips. Strain RFF231 (rpoH1rpoH2 deletion), carrying either Ca. Liberibacter asiaticus rpoH (locus tag = CLIBASIA_02490, codon optimized for expression in Sinorhizobium meliloti) or Sinorhizobium meliloti rpoH1 (locus tag = SMc00646) was compared to strain RFF231, carrying the empty vector, pSRKGm (Khan et al. 2008, Appl Environ Microbiol 74:5053-5062)

本研究对分别表达亚洲韧皮部杆菌（Candidatus Liberibacter asiaticus）rpoH或苜蓿中华根瘤菌（Sinorhizobium meliloti）rpoH1的苜蓿中华根瘤菌rpoH1rpoH2缺失突变株（RFF231；Lang等，2018，mSphere 3:e00454-18）的转录组进行了表征。
实验设计概述：采用定制化Affymetrix基因芯片对苜蓿中华根瘤菌菌株进行基因表达谱分析。将携带亚洲韧皮部杆菌rpoH（基因座标签：CLIBASIA_02490，已针对苜蓿中华根瘤菌表达完成密码子优化）或苜蓿中华根瘤菌rpoH1（基因座标签：SMc00646）的RFF231菌株（rpoH1rpoH2缺失株），与携带空载体pSRKGm的RFF231菌株（Khan等，2008，Appl Environ Microbiol 74:5053-5062）进行对照比较。