Perl script for functional annotations

Hit-desc.pl - Perl script for adding functional annotations to contigs using BLAST output (-outfmt 6). Note: This script uses regular expressions that are specific to targeting the accession numbers of our contigs and those that were used for BLAST searches. It is to be used as a reference for writing similar scripts for similar purposes but will not work as is for datasets other than our own. Requires an installation of BioPerl. Running too much BLAST output at once will kick you off of the NCBI server, so restrict the number of queries to ~500.

Hit-desc.pl 是一款Perl脚本，可依托BLAST输出（格式为 -outfmt 6）为重叠群（contigs）添加功能注释。注意：本脚本所使用的正则表达式仅针对本研究中所用重叠群以及BLAST搜索所用序列的登录号（accession number）设计，仅可作为编写同类功能脚本的参考范本，无法直接适配除本研究数据集之外的其他数据集。运行本脚本需预先安装BioPerl。一次性处理过多BLAST输出会导致被NCBI服务器封禁，因此需将查询数量限制在约500条以内。